AO|2M received an Amsterdam Cardiovascular Sciences (ACS) equipment grant for expanding its research capabilities with a spectral detector on the Nikon A1R+ HD platform.
The Nikon A1 DUS spectral detector has 32 channels, allowing acquisition of a 512 px image in 0.6 seconds. Moreover, 512 x 32-pixel images can be captured at 24 fps. Using the unmixing capabilities of the A1 DUS spectral detector, live unmixing of closely overlapping fluorescence spectra becomes possible. It has been demonstrated that two fluorophores with identical excitation spectra and only 5nm difference in peak-emission can be unmixed. This feature will allow researchers to investigate more complex protein-protein interactions in both fixed and living samples.